Long pcr master mix contains a small amount of dna polymerase with 3. Taq polymerase for robust pcr with and directtogel convenience. Promega products are warranted to meet or exceed the stated specifications. Gotaq g2 dna polymerase is supplied with 5x green gotaq reaction buffer and 5x colorless gotaq reaction buffer. The pcr master mix is designed for routine endpoint pcr for dna amplicons in the range of 0. Gotaq dna polymerase m300 protocolpdf 207 kb english.
Scientists realized that thermostable heatstable dna polymerases would be needed for pcr to work efficiently. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Purification of taq dna polymerase for 1 liter culture modified from the protocol presented in f. Dnafree dna polymerases thermo fisher scientific us. Second generation, highperformance gotaq g2 dna polymerase with mgfree buffers. Uncompromised specificity of platinum taq dna polymerase, dna free, as compared to competitor. Taq dna polymerase is a thermostable dna polymerase that possesses a 5. Convenient bundles containing taq polymerase and reagents for pcr.
Taq dna polymerase definition of taq dna polymerase by. Gotaq g2 master mixes are premixed readytouse solutions containing gotaq g2 dna polymerase, dntps, mgcl2 and reaction buffers at optimal concentrations for efficient amplification of a wide range of dna templates by pcr. It has a 53 dna polymerase activity and a 53 exonuclease activity see figure. Taq dna polymerase with standard taq mgfree buffer neb. Mg free buffers for pcr optimization with directtogel convenience. A protocol for gotaq flexi dna polymerase which provides robust amplification equal to and in some cases superior to conventional taq dna polymerase. Recombinant taq is a 94kda enzyme with an optimal 53 polymerase activity between 7580 c in the presence of a magnesium cofactor.
Pluthero 1993 rapid purification of highactivity taq dna polymerase. The following guidelines are provided to ensure successful pcr using nebs taq dna polymerase. Promegas pcr systems, enzymes and reagents are proven in pcr to ensure reliable, high performance results. Gotaq long pcr master mix technical manualpdf promega. It comes in a variety of formats designed to provide maximum flexibility, control and convenience. Pcr master mix includes nuclease free water and pcr master mix, 2x. The proven robust amplification using gotaq polymerase is now available for longrange pcr up to 30kb gdna. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 9495 degrees c for two minutes. Allows optimization of mgcl 2 concentration and facilitates directtogel analysis of pcr products. Commercial producers have sinced cloned and expressed the enzyme in e.
Gotaq long pcr master mix lane p outperforms other leading long pcr products lanes t, n, l and s for amplification of a 17. Gotaq dna polymerase is a proprietary formulation of taq dna polymerase that gives robust amplification equal to and in some cases superior to that of standard taq dna polymerase. Simplify your reactions and reduce the possibility of contamination by adding nucleotides as a mix. Buffer choice we recommend using the 5x green gotaq reaction buffer in any amplification reaction.
The supplied 5x green and colorless flexi reaction buffers do not contain magnesium. Pfu dna polymerase is a highfidelity, thermostable enzyme of approximately 90kda isolated from pyrococcus furiosus. Mtp taq dna polymerase is a recombinant thermostable enzyme from thermus aquaticus expressed in e. Download a pdf containing pricing for our full product list. Promegas sole obligation and the customers sole remedy is limited to replacement of products free of charge in the event products fail to perform as warranted. Performance of platinum taq dna polymerase, dna free blue curves, and promega gotaq mdx hot start polymerase green curves in qpcr assays were evaluated using primers targeting e. Each promega product is shipped with documentation stating specifications and other technical information.
Taq polymerase, being thermostable, proved ideal for pcr. The polymerase chain reaction pcr was developed by chemist kary mullis in the 1980s, as a means to make many copies of dna fragments. Platinum ii taq hotstart dna polymerase is the next generation hotstart dna polymerase, newly engineered for rapid, robust performance. Gotaq green master mix product information promega corporation. Gotaq g2 dna polymerase reliably amplifies a wide range of pcr. If you are not completely satisfied with any promega pcr product, we will send a replacement or refund your account. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. Our teams are in regular contact with suppliers and distributors worldwide to manage inventory of raw materials to ensure uninterrupted availability.
The experiments were performed at the molecular diagnostic division of bhat biotech india p ltd. Pcr protocol for taq dna polymerase with standard taq mg free buffer m0320 overview. It is supplied with 10x standard taq mg free reaction buffer and mgcl 2. Gotaq dna polymerase contains gotaq dna polymerase, 5x green gotaq reaction buffer and 5x colorless gotaq reaction buffer. The enzyme is a fulllength form of taq dna polymerase that exhibits 53 exonuclease activity. Enhanced performance over standard taq dna polymerase.
Platinum ii taq hotstart dna polymerase thermo fisher. Highlevel expression and purification of dna and dnase. Promega corporation 2800 woods hollow roadmadison, wi 537115399 u. Mtp taq dna polymerase taq dna polymerase, free of dna. Pcr master mix is a premixed, readytouse solution containing taq dna polymerase, dntps, mgcl 2 and reaction buffers at optimal concentrations for efficient amplification of dna templates by pcr. Taq dna polymerase is a thermostable enzyme that synthesizes dna from singlestranded templates in the presence of dntps and a primer. Gotaq flexi dna polymerase is a proprietary formulation of taq dna polymerase that gives robust amplification equal to and in some cases superior to that of standard taq. Gotaq hot start master mixes are allinone master mixes just add template and primers that contain the highperformance gotaq dna polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 9495c for two minutes.
Taq dna polymerase is an enzyme widely used in pcr 2. Day 1 start 3ml overnight culture of taq from glycerol stock in lbamp 75ngul day 2 add 1ml of overnight culture to 1l of lbamp. Gotaq hot start polymerase product information promega. Both buffers contain mgcl 2 at a concentration of 7. Product components and storage conditions product size cat. Q5 highfidelity dna polymerase offers high fidelity 280x higher than taq. Our products are backed by a worldwide team of technical support scientists. Gotaq dna polymerase product information promega corporation. Tsongalis, in diagnostic molecular pathology, 2017.
Toll free in the usa 8003569526 telephone 6082744330 usage information b. Promega s pcr systems, enzymes and reagents are proven in pcr to ensure reliable, high performance results. The glycerol free product formulation is further purified to remove glycerol, making the enzyme suitable for further manufacture processing and lyophilization. Set up pcr in less than a minute with convenient master mixes. It is supplied with 10x standard taq reaction buffer, which is detergent free and designed to be compatible with existing assay systems. The enzyme consists of a single polypeptide with a molecular weight of 94 kda. The 5x green gotaq reaction buffer has two dyes a blue dye and a yellow dye that separate during electrophoresis to show migration progress. The 5x gotaq green and colorless reaction buffers supplied with gotaq dna polymerase contain mgcl 2 at a concentration of 7. The enzyme is a taq dna polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. Native taq dna polymerase with roomtemperature setup for hotstart pcr. Taq polymerase is a thermostable dna polymerase i named after the thermophilic eubacterial microorganism thermus aquaticus, from which it was originally isolated by chien et al. Shop online for a wide selection of promega gotaq dna polymerase taq dna polymerase. Gotaq g2 is a fulllength, recombinant taq polymerase supplied with buffers designed for enhanced amplification.
Gotaq long pcr master mix contains the highperformance gotaq hot start polymerase in a specially formulated mixture with a proprietary thermal stable proofreading polymerase. This enables hotstart pcr, where polymerase activity is eliminated or minimized at temperatures below 70 degrees c. It is frequently used in the polymerase chain reaction pcr, a method for greatly amplifying the quantity of short segments of dna. Gotaq flexi dna polymerasea,b is supplied in a proprietary. Gotaq dna polymerase comes in a variety of formulations to give. Toll free in the usa 8003569526 telephone 6082744330. Gotaq dna polymerase is supplied in 2x green gotaq reaction buffer. The enzyme replicates dna at 75c, catalyzing the polymerization of nucleotides in the 5. Pcr the polymerase chain reaction pcr is a powerful and sensitive technique for dna amplification 1. Base misincorporations that may occur during polymerization. Pcr protocol for taq dna polymerase with standard taq mg. The enzyme is a fulllength form of taq dna polymerase that exhibits 53.
Gotaq dna polymerase is a proprietary formulation of taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard taq. Provided with green and colorless reaction buffers. This lowtemperature polymerase activity is the basis for nonspecific amplification associated with mispriming events that. Secondgeneration, highperformance gotaq g2 dna polymerase with mgfree buffers. Gotaq dna polymerasea,b is a taq dna polymerase supplied in a proprietary formulation.
Gotaq mdx dna polymerase is a fulllength form of taq dna polymerase that exihibits 5. Highperformance taq dna polymerase, nucleotides dntps, buffers and master mixes provide increased reliability and consistency for routine endpoint pcr. Our teams are in regular contact with suppliers and distributors worldwide to manage inventory of raw materials to ensure continued availability. The enzyme is a fulllength form of taq dna polymerase that exhibits 5. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 6082744330 fax 6082772516 3 tm379 revised 420 2. Gotaq dna polymerase is a proprietary formulation of taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard taq the 5x gotaq green and colorless reaction buffers supplied with gotaq dna polymerase contain mgcl 2 at a concentration of 7. Pcr master mix is a readytouse 2x solution containing taq dna polymerase, dntps, mgcl 2 and reaction buffers at optimal concentrations for efficient pcr amplification of dna templates. Gotaq flexi dna polymerase m829 protocolpdf 207 kb english. The onetaq reaction buffers and high gc enhancer have been formulated for robust yields with minimal optimization, regardless.
We recommend that investigators starting new projects use platinum ii taq dna polymerase so that they can benefit from its superior performance, summarized in the table below. First isolated from the bacterial thermophile thermus aquaticus, the taq polymerase has become the staple enzyme in molecular biology. Native taq dna polymerase with 5x reaction buffers. Gotaq g2 dna polymerase product informationpdf 191 kb english. Maximum flexibility, control and convenience for optimizing pcr. The gotaq g2 hot start master mixes are readytouse mixes containing all necessary components gotaq g2 hot start polymerase, buffer, dntps and optimized magnesiumyou only need to add primer and template, and go. All components should be mixed and spun down prior to pipetting. Secondgeneration, highperformance gotaq g2 dna polymerase with mg free buffers. Pcr amplification of bacterial dna with low contamination using mtp taq dna polymerase. Pcr protocol for taq dna polymerase with standard taq buffer m0273.
558 105 1603 160 800 667 441 1190 1032 826 908 865 191 1009 1011 40 736 96 293 1452 194 1220 1024 1550 482 551 1273 772 1163 672 909 548 1135 437 688 1476 934 1401 1298 1105 1300